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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/10/2004 |
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Data da última atualização: |
04/11/2004 |
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Autoria: |
OLIVEIRA, R. P. de; RADMANN, E. B. |
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Título: |
Genetic characterization of new varieties and hybrids of citrus table fruit through isoenzymes. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Londrina, v. 3, n. 1, p. 77-82, Mar. 2003. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to establish isoenzymatic patterns to genetically characterize the orange varieties 'Lane Late', 'Navalete', 'Navelina' and 'Salustiana', mandarin varieties 'Clemenules', 'Marisol' and 'Satsuma Okitsu', and the hybrids 'Nova' and 'Ortanique'. The following isoenzymatic systems were utilized: alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (APS), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), 6-phosphogluconate dehydrogenase (6-PGDH), glutamate oxaloacetate transaminase (GOT), isocitrate dehydrogenase (IDH), leucine aminopeptidase (LAP), malate dehydrogenase (MDH), peroxidase (PRX), sorbitol dehydrogenase (SDH), superoxidase dismutase (SOD) and shikimate dehydrogenase (SKDH). Proteins were extracted from leaves and the isoenzymatic polymorphisms were analyzed by polyacrylamide fel electrophoresis. The PGM isoenzyme showed the highest differentiation among the genetic materials tested. The banding profiles observed for PGM, SKDH and EST were satisfactory to characterize the mandarin varieties and hybrids studied. However, no polymorphic bands were found in sweet oranges with the 14 isoenzymes tested. |
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Palavras-Chave: |
Biochemical markers; citrus crop; genotype identification; Identification; Orange. |
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Thesagro: |
Laranja. |
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Thesaurus Nal: |
electrophoresis; genotype. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01840naa a2200229 a 4500
001 1212659
005 2004-11-04
008 2003 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, R. P. de
245 $aGenetic characterization of new varieties and hybrids of citrus table fruit through isoenzymes.
260 $c2003
520 $aThe aim of this study was to establish isoenzymatic patterns to genetically characterize the orange varieties 'Lane Late', 'Navalete', 'Navelina' and 'Salustiana', mandarin varieties 'Clemenules', 'Marisol' and 'Satsuma Okitsu', and the hybrids 'Nova' and 'Ortanique'. The following isoenzymatic systems were utilized: alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (APS), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), 6-phosphogluconate dehydrogenase (6-PGDH), glutamate oxaloacetate transaminase (GOT), isocitrate dehydrogenase (IDH), leucine aminopeptidase (LAP), malate dehydrogenase (MDH), peroxidase (PRX), sorbitol dehydrogenase (SDH), superoxidase dismutase (SOD) and shikimate dehydrogenase (SKDH). Proteins were extracted from leaves and the isoenzymatic polymorphisms were analyzed by polyacrylamide fel electrophoresis. The PGM isoenzyme showed the highest differentiation among the genetic materials tested. The banding profiles observed for PGM, SKDH and EST were satisfactory to characterize the mandarin varieties and hybrids studied. However, no polymorphic bands were found in sweet oranges with the 14 isoenzymes tested.
650 $aelectrophoresis
650 $agenotype
650 $aLaranja
653 $aBiochemical markers
653 $acitrus crop
653 $agenotype identification
653 $aIdentification
653 $aOrange
700 1 $aRADMANN, E. B.
773 $tCrop Breeding and Applied Biotechnology, Londrina$gv. 3, n. 1, p. 77-82, Mar. 2003.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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